Human CTGF ELISA kit (ab261851) (2024)

Overview

• Product name

  Human CTGF ELISA kit
  See all CTGF kits

• Detection method

  Colorimetric

• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  Serum	8			5.8%

  Inter-assay

  Sample	n	Mean	SD	CV%
  Serum	3			7.4%

• Sample type

  Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma

• Assay type

  Sandwich (quantitative)

• Sensitivity

  64 pg/ml

• Range

  93.75 pg/ml - 6000 pg/ml

• Recovery

  Sample specific recovery

  Sample type	Average %	Range
  Serum	99	97% - 101%
  Cell culture media	110	108% - 111%
  Hep Plasma	129	127% - 130%
  EDTA Plasma	121	117% - 124%
  Cit plasma	100	94% - 105%

  See Also

  MRTF-A controls vessel growth and maturation by increasing the expression of CCN1 and CCN2[citation report] CCN2-induced lymphangiogenesis is mediated by the integrin αvβ5–ERK pathway and regulated by DUSP6Cell–cell communication in kidney fibrosis

• Assay time

  1h 30m

• Assay duration

  One step assay

• Species reactivity

  Reacts with: Human
  Does not react with: Cow

• Product overview

  Human CTGF ELISA kit (ab261851) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CTGF protein in cell culture media, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CTGF with 64 pg/ml sensitivity.

  
  

  SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

  
  

  - Single-wash protocol reduces assay time to 90 minutes or less
  - High sensitivity, specificity and reproducibility from superior antibodies
  - Fully validated in biological samples
  - 96-wells plate breakable into 12 x 8 wells strips

  
  

  A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

• Notes

  Human connective tissue growth factor (CTGF), also known as CCN2, and Insulin-like growth factor-binding protein 8, is a matricellular protein encoded by the gene CTGF. Along with CYR61 (CCN1) and NOV (CCN3), CTGF belongs to the CCN family of extracellular matrix-associated heparin binding proteins. The CCN proteins contain IGFBP, Von Willebrand type C repeats, and thrombospodin type 1 repeat domains. CTGF has multiple functions including promotion proliferation and differentiation of chondrocytes; mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells, and enhances fibroblast growth factor-induced DNA synthesis.

• Platform

  Pre-coated microplate (12 x 8 well strips)

Properties

• Storage instructions

  Store at +4°C. Please refer to protocols.

• Components	1 x 96 tests	1 x 384 tests
  10X Human Human CTGF Capture Antibody	1 x 600µl	1 x 600µl
  10X Human Human CTGF Detector Antibody	1 x 600µl	1 x 600µl
  10X Wash Buffer PT (ab206977)	1 x 20ml	1 x 20ml
  384 well CaptSure™ microplates	0 x 0 unit	1 unit
  Antibody Diluent 5BI	1 x 6ml	1 x 6ml
  Human CTGF Lyophilized Recombinant Protein	2 vials	2 vials
  Plate Seal	1 unit	1 unit
  Sample Diluent 25BP	1 x 20ml	1 x 20ml
  Sample Diluent NS (ab193972)	1 x 12ml	0 x 0ml
  SimpleStep Pre-Coated 96-Well Microplate (ab206978)	1 unit	0 x 0 unit
  Stop Solution	1 x 12ml	2 x 12ml
  TMBDevelopment Solution	1 x 12ml	2 x 12ml

• Research areas

  • Cardiovascular
  • Angiogenesis
  • Growth Factors
  • Other

  • Immunology
  • Secreted Molecules
  • CCN Family

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • 384-well SimpleStep ELISA

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • SSE kits

• Function

  Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.

• Tissue specificity

  Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.

• Sequence similarities

  Belongs to the CCN family.
  Contains 1 CTCK (C-terminal cystine knot-like) domain.
  Contains 1 IGFBP N-terminal domain.
  Contains 1 TSP type-1 domain.
  Contains 1 VWFC domain.

• Cellular localization

  Secreted > extracellular space > extracellular matrix. Secreted.

• Information by UniProt

• Alternative names

  • CCN 2
  • CCN family member 2
  • CCN2

  see all

• Database links

  • Entrez Gene: 1490 Human
  • Omim: 121009 Human
  • SwissProt: P29279 Human
  • Unigene: 410037 Human

• Sandwich ELISA - Human CTGF ELISA kit (ab261851)

  Example of human CTGF standard curve in Sample Diluent 25BP in 96-well vs. 384-well plate.

• Sandwich ELISA - Human CTGF ELISA kit (ab261851)

  Example of human CTGF standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

• Example of human CTGF standard curve in Sample Diluent 25BP.

  Example of human CTGF standard curve in Sample Diluent 25BP. The CTGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

• Interpolated concentrations of native CTGF in human serum and plasma samples.

  Interpolated concentrations of native CTGF in human serum and plasma samples. The concentrations of CTGF were measured in duplicates, interpolated from the CTGF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 5%, plasma (heparin) 5% and plasma (EDTA)10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CTGF concentration was determined to be 13,695 pg/mL in serum, 10,342 pg/mL in plasma (citrate) and 9,035 pg/mL in plasma (heparin) 5% and 10,298 pg/mL plasma (EDTA)10%.

• Serum from eight individual healthy human gender donors was measured in duplicate.

  Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CTGF concentration was determined to be 18.8 ng/mL with a range of 11.32 – 25.5 ng/mL.

• Sandwich ELISA - Human CTGF ELISA kit (ab261851)

  SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

• Sandwich ELISA - Human CTGF ELISA kit (ab261851)

  To learn more about the advantages of recombinant antibodies see here.

• Sandwich ELISA - Human CTGF ELISA kit (ab261851)

  To learn more about the advantages of SimpleStep ELISA^® kits see here.

• Protocol Booklet

Click here to view the general protocols

• SDS download

  Country/Region

  Language

  

• Datasheet download

  Download

ab261851 has been referenced in 4 publications.

• Rühle A et al. The value of plasma hypoxia markers for predicting imaging-based hypoxia in patients with head-and-neck cancers undergoing definitive chemoradiation. Clin Transl Radiat Oncol 33:120-127 (2022). PubMed: 35243023
• Yang M et al. Elevated Serum IGFBP-2 and CTGF Levels Are Associated with Disease Activity in Patients with Dermatomyositis. Dis Markers 2022:9223883 (2022). PubMed: 35356065
• Johansson M et al. Data Mining Identifies CCN2 and THBS1 as Biomarker Candidates for Cardiac Hypertrophy. Life (Basel) 12:N/A (2022). PubMed: 35629393
• Rovira E et al. U1A is a positive regulator of the expression of heterologous and cellular genes involved in cell proliferation and migration. Mol Ther Nucleic Acids 28:831-846 (2022). PubMed: 35664701